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Centrifuge the homogenate at 9000 × g for 10 min, recover the supernatant, and transfer it to a new glass tube with glass pipette Wash the supernatant with 02 volumes (eg, 2 ml for 10 ml) of water Vortex the sample for 30 s and then centrifuge at 1000 × g for 5 min to separate the two phas
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